AkaLumine hydrochloride is a fluorescein analog with a Km value of 2.06 μM for recombinant fluorescent protein.

存储：store at low temperature,keep away from direct sunlight　

          Powder: -20°C for 3 years | In solvent: -80°C for 1 year

别名 ：AkaLumine hydrochloride (1176235-08-7 free base), AkaLumine hydrochloride
分子量：338.85
分子式：C16H19ClN2O2S
CAS No.  ：2558205-28-8

溶解度：
DMSO: 45.0 mg/mL (132.8 mM), Sonication is recommended.
H2O: 45.0 mg/mL (132.8 mM);, Sonication is recommended.

靶点活性：     Recombinant Fluc protein:2.06 μM
体外活性：     In both LLC/luc and MDA-MB-231/Luc cells exposed to AkaLumine hydrochloride, the signal achieves its peak intensity at lower concentrations (starting from 2.5 μM). Conversely, the bioluminescence produced by D-luciferin and CycLuc1 exhibits a continuous rise with increasing concentrations, without reaching an apparent saturation point, even at the highest concentration of 250 μM.[1]
体内活性 ：    AkaLumine hydrochloride-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, exhibit a 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with the experimental control group. In the inverse order at an 8 h interval. AkaLumine hydrochloride displays about a fourfold increase in signals from lung metastasis compared with the experimental control group.[1]

参考文献
1. Kuchimaru T, et al. A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat Commun. 2016 Jun 14;7:11856.
2. Ogawa H, et al. Absorption Spectra for Firefly Bioluminescence Substrate Analog: TokeOni in Various pH Solutions. Photochem Photobiol. 2021;97(5):1016-1022.
3. Zambito G, et al. Evaluating Brightness and Spectral Properties of Click Beetle and Firefly Luciferases Using Luciferin Analogues: Identification of Preferred Pairings of Luciferase and Substrate for In Vivo Bioluminescence Imaging. Mol Imaging Biol. 2020;22(6):1523-1531.
4. Hendrickx S, et al. Comparison of Bioluminescent Substrates in Natural Infection Models of Neglected Parasitic Diseases. Int J Mol Sci. 2022;23(24):16074.